Quantum Dot Conjugated Magnetic Nanoparticles for Targeted Drug Delivery and Imaging
Indu Venugopal1, Sebastian Pernal1, Taylor Fusinatto1,2, David Ashkenaz1,3, Andreas Linninger1*
1University of Illinois at Chicago, Department of Bioengineering, Laboratory for Product and Process Design
2NSF-RET fellow from Bessie Rhodes Magnet School, Science Department
3NSF-RET fellow from Innovations High School, Science Department
*Corresponding author. E-mail: email@example.com
Received: Dec. 12, 2015; Accepted: Feb. 18, 2016; Published: Mar. 12, 2016.
Citation: Indu Venugopal, Sebastian Pernal, Taylor Fusinatto, David Ashkenaz and Andreas Linninger. Quantum Dot Conjugated Magnetic Nanoparticles for Targeted Drug Delivery and Imaging. Nano Biomed. Eng. 2016 8(1), 24-38. DOI: 10.5101/nbe.v8i1.p24-38.
Nanotechnology is being increasingly applied for developing drug delivery options for specific treatments. Magnetic nanoparticles have drawn attention as drug delivery vehicles due to their stability, biocompatibility and ability to be non-invasively guided to desired target areas using magnetic fields. In this paper, we describe a new delivery vehicle for magnetic drug targeting. In magnetic drug targeting, drug functionalized magnetic nanoparticles are guided and localized at specific sites using external magnetic fields. Magnetic nanoparticles act as contrast agents for magnetic resonance imaging. However, it cannot be visualized via this technique during drug delivery. This is between magnetic fields used for imaging and delivery can interference with each other. Our laboratory has synthesized a magnetic drug targeting vehicle conjugated with quantum dots that can be imaged during the drug delivery process with in vivo imaging techniques such as fluorescence molecular tomography. These nanocomposites can be used as drug delivery vehicles for the central nervous system, where drug targeting is especially difficult and minimizing side effects is critical.
Keywords: Fluorescent nanoparticles; Magnetic nanoparticles; Magnetic drug targeting; Drug delivery
Along with the discovery and development of therapeutics molecules, one of the main challenges for disease treatment is the difficulty to deliver them to target sites within the body. Drug delivery to specific organs or tissues, especially to the Central Nervous System (CNS), poses significant challenges such as achieving high efficacy while evading side effects [1, 2]. Currently, the lack of specificity towards the target site generates the need for using high drug dosages to attain required therapeutic effect. However, this elevates the risk of toxicity in non-targeted regions, leading to unwanted side effects. For example, high doses of chemotherapeutic drugs are known to cause unacceptable systemic toxicity [3-6]. A significant advancement has been the development of nanoparticles (NPs) for delivering drugs. Variation in their biochemistry and material properties can create innovative therapeutic options for site-specific drug delivery, which takes advantage of interactions between target molecules with cellular and sub- cellular structures [7-12]. Targeted delivery reduces drug dosage and minimizes systemic toxicity, while maintaining therapeutic efficacy. The large surface area to volume ratio of nanoparticles, provides an ideal platform for drug loading and surface modifications for site specific targeting [13-18]. The small size of the NPs allows them to circumvent some of the most impenetrable barriers of the human body. For example, the blood brain barrier (BBB), which is formed by a continuous layer of endothelial cells joined together by tight junctions (zonulae occludens) surrounding blood vessels [19, 20], severely restricts access of large therapeutic molecules to the brain and spinal cord tissue. Previously, NP systems have been utilized to by-pass the BBB for treatment of CNS diseases like gliomas [21-23]. NP-based drug delivery systems also offer other benefits such as increased drug bioavailability [24-26], higher stability for treatments involving controlled release of drugs [27-29], effective delivery of poorly water soluble drugs and co-delivery of two or more agents rendering multi-functionality [30-32]. Magnetic nanoparticles (MNPs) have drawn interest for their intrinsic magnetic property, which is advantageous for targeted drug delivery and biomedical imaging. MNPs lesser than 30 nm in diameter are superparamagnetic; a property that enables them to get temporarily magnetized in presence of a magnetic field and lose all magnetization once the field is removed. This property prevents unwanted agglomeration which is important for targeted delivery . MNPs are also used as a contrast agent for magnetic resonance imaging (MRI) due to its ability to reduce the T2 relaxation time of water molecules [34, 35]. More importantly, MNPs can be utilized for magnetic drug targeting (MDT), which is an active drug targeting method. During MDT, drugfunctionalized magnetic nanoparticles are administered into a body fluid, which can then be localized and concentrated at specific target sites using external magnetic fields. The active drug desorbs from the nanoparticles and initiates therapeutic activity. The most notable benefit of MDT is the ability to implement local drug action by providing a non-invasive, drug/ligand independent targeting technique. Although MNPs have been commonly administered via the blood stream, recent applications have targeted the CNS by intrathecal administration of MNPs into the subarachnoid space [36, 37]. Several research groups have developed MNPs for exploiting their properties as a contrast agents for MRI [38-42]. Other labs have accomplished site specific targeting of drug loaded MNPs using appropriate ligands, proteins and other molecules . Enhanced permeation and retention effect aided in accumulating MNPs in target sites rather than relying on MDT [44-46]. Despite these achievements, imaging MNPs via MRI during MDT remains difficult, because the magnetic fields used to generate images interfere with the magnetic fields needed for MDT. Therefore, there is a need for an MDT delivery vehicle that can be imaged in vivo without resorting to MRI. In this paper, we present a nanovehicle that can be specifically targeted via MDT and have its distribution tracked during in vivo delivery. This vehicle comprises of a gold-coated magnetite nanoparticle (Fe3O4@AuNP) conjugated to cadmium telluride quantum dots (CdTe QDs). It offers three major benefits: (i) The vehicle can be targeted, localized and concentrated at specific sites within tissues or organs; magnetite (Fe3O4) core of these nanoparticles allows steering under the influence of externally placed magnets. (ii) The outer gold shell of the vehicle can be loaded with therapeutic molecules. Drugs that have sulfur-containing functional groups show a strong affinity to bind to gold surfaces [47,48], which enables conjugation of many active agents with relative ease.(iii) The cadmium telluride quantum dots (CdTeQDs) can be imaged in vivo during delivery due to strong fluorescence emission. Commercially available imaging modalities utilizing fluorescence molecular tomography (FMT) are capable of imaging this nanocomposite in vivo, due to the strong fluorescence emission by the quantum dots (QDs). QDs are nanometer sized highly fluorescent particles made of semiconductor materials, which do not photobleach easily. QDs retain their strong fluorescence even after conjugation to the nanoparticles, when compared to organic molecules such as rhodamine and fluorescein [49, 50]. QDs also have broader excitation spectra (high absorption) and a narrow, sharp emission peak , which results in a brighter emission and a higher signal to noise ratio. This paper presents a novel QD-MNP drug delivery vehicle and is organized as follows. Methods section describes the synthesis and characterization results of our fluorescent nanocomposite and its individual components. The results section demonstrates cellular uptake, in vitro MDT and in vivo visualization of the nanocomposites. The paper closes with a discussion of our experimental results and conclusions.
Materials and Methods
The synthesis method of each of the individual components-gold coated magnetite nanoparticles (Fe3O4@Au MNPs), cadmium telluride quantum dots, and conjugation into a single nanocomposite has been described separately. Sodium hydroxide (NaOH) solution, ethanol (molecular biology grade) and 3-mercaptopropionic acid (HSCH2CH2CO2H) was purchased from Sigma Aldrich (MO, USA). 1 M TX-100 solution and trisodium citrate dihydrate (HOC(COONa) (CH2COONa)2·2H2O) was purchased from Fisher Scientific (NJ, USA). Hydrogen tetrachloroaurate (III) hydrate (HAuCl4), sodium borohydride (NaBH4) and sodium tellurite was purchased from Alfa Aesar (MA, USA). All other chemicals were purchased from Acros Organics (NJ, USA). Nanopure® water (Barnstead Nanopure lab water system) was used for all procedures described below. This section starts with a detailed description for the synthesis of the magnetic nanocomposites (§2.1, §2.2 and §2.3). Then cell-based experiments for proving biocompatibility and applicability of the nanocomposites in the CNS are described (§2.4). An in vivo imaging experiment illustrates imaging ability of the vehicle. Finally, an MDT experiment demonstrates the magnetic targeting capability of our nanocomposites (§2.5, §2.6 and §2.7).
Synthesis of gold coated magnetite nanoparticles
The Fe3O4 cores were synthesized by a coprecipitation technique, well described by Mandal et al. , and various other articles, using ferrous and ferric salts to form Fe3O4 [53-55].
Preparation of the iron stock solution
1.543g of ferric ammonium sulfate [NH4Fe(SO4)2·12H2O] and 0.6275 g of ferrous ammonium sulfate [(NH4)2Fe(SO4)2·6H2O] were dissolved in 25 mL of 0.40 M aqueous sulfuric acid solution to make the iron stock solution. The molar concentrations of the two iron salts in the solution were 0.128 M and 0.064 M respectively.
Synthesis of magnetite nanoparticle cores
1.5 mL of 1 M TX-100 solution was added to 250 mL of 1 M NaOH solution and heated to 75 °C in a water bath. 10 mL of the iron stock solution was added dropwise into this solution with vigorous nonmagnetic stirring. After complete addition of the stock solution, stirring was continued for another 20 minutes (min). During the entire procedure, the temperature was maintained at 75 °C. Black Fe3O4 nanoparticle cores were obtained after settling followed by magnetic decantation to remove the supernatant. The particles were washed multiple times with water to remove the excess surfactant. The nanoparticles were then resuspended in water.
Coating magnetite cores with gold
The solution containing the prepared Fe3O4 cores was sonicated for 15 min before placement in the water bath heated to 60 °C. While slowly stirring the solution with a non-magnetic stirrer, 0.5 g of D(+)- glucose was added. After 5 min 110 μL of 1 g mL-1 aqueous solution of hydrogen tetrachloroaurate (III) hydrate was added dropwise. The temperature of the water bath was maintained at 60 °C and the solution was slowly stirred for 1 h in the dark. The color of the Fe3O4 nanoparticle cores changed to a brownred during the gold coating process. The solution was magnetically decanted and washed several times before being resuspended in water to a concentration of 0.17 mg mL-1.
Synthesis of CdTe quantum dots
The microwave based synthesis procedure was similar to Duan et al.  with modifications made to the microwaving procedure. The procedure yielded QDs in the 2-6 nm size range.
Preparation of the cadmium chloride stock solution
0.4 mL of 0.04 M of cadmium chloride solution was diluted with 42 mL of water to produce the stock solution.
Preparation of the Cd-MPA-Te solution
100 mg of trisodium citrate dihydrate, 4 mL of 0.01 M sodium tellurite solution, 119 mg of 3 mercaptopropionic acid and 50 mg of sodium borohydride were added to the cadmium chloride stock solution under magnetic stirring. The molar ratio of Cd:MPA:Te in the solution was 1:7:0.25. The pH of the solution was maintained at 8.
Synthesis of the QDs by using microwaving technique
10 mL of the solution was placed in a 60 mL teflondigestion vessel (Savillex corporation, MN, USA). The QDs were synthesized with varying temperatures and microwaving time durations (89 °C-130 °C and 1-5 min) to produce QDs of different colors. The power of the microwave device (Samsung, 1.05 Kw Micro Wave) was set at 900W. The QD samples were allowed to cool to room temperature prior to further examination.
Synthesis of quantum dot conjugated gold coated magnetite nanocomposites (QDFe3O4@Au MNPs)
1 mL of both the Fe3O4@Au MNPs and CdTe QD solutions (0.17 mg mL-1) was added to 20 mL of ethanol in a glass container. The glass container was kept in the dark for 48 h under constant slow stirring. This allowed for conjugation of the QDs to the gold coating of the Fe3O4@Au MNPs to form the fluorescent nanocomposites. The excess ethanol was removed post conjugation by magnetic decantation. To remove any excess QDs, the solution was washed with water to remove the free-floating water soluble QDs, followed by magnetic decantation to separate the nanocomposites. This process was repeated several times to remove all the free QDs from the solution, which can be confirmed by absence of fluorescence emission from the supernatant obtained on magnetic decantation. The washed nanocomposites are resuspended in pure water to obtain the desired concentration.
Cell transfection experiment using C6 glioma cells
In order to produce a cytotoxic effect in diseased target cells, nanocomposites need to be internalized. Therefore, the uptake of fluorescent nanocomposites into rat C6 glioma cells was studied in a transfection experiment. Cells were maintained in medium containing Dubelcco’s Modified Eagle Medium (DMEM) and 10% fetal bovine serum with 1% antibiotics. Various concentrations of the nanocomposites were prepared using the cell growth medium as diluent. 1mL of this nanocomposite solution was applied to cells cultured in a 12-well plate. After incubation for 5 hours, cells were fixed with a 4% paraformaldehyde solution, while protecting it from light exposure to avoid any unwanted loss of fluorescence. Phalloidin-CruzFlour488 conjugate (Santa Cruz Biotechnology, 1:1000 solution in PBS) was applied onto the fixed sample for 60 min. This was used to stain the cells’ actin cytoskeleton. After rinsing phalloidin completely, DAPI (Biotium, 1:4000 solution in PBS) was applied for 5 min to stain chromatin in the nuclei of the cells. Cells were imaged using a normal Zeiss Axioskope fluorescence microscope. The cellular uptake of the nanocomposites was quantified using Image J software . The quantification was performed by compiling three fluorescence images corresponding to the DAPI, nanocomposite and phalliodin stains (Fig. 4(a), 4(b), 4(c)). Phalloidin stains the actin cytoskeleton, marking the cell boundaries, and appears green; DAPI stains the nucleus, assisting in cell counting, and appears blue; and the nanocomposites appear red. Using the compiled image, only the portion of the image containing cells is selected and Image J was used to quantify the average amount of red intensity per pixel. The average red intensity per pixel in the background of the composite image is subtracted from the average within the cell boundary. This final value provides arelative estimate of the nanocomposite uptake.
Delivery of the nanocomposites into the intrathecal space of a Sprague Dawley rat
12-week old Sprague Dawley rats were used for this procedure. All animal experiments were approved by the Animal Care and Use Committee at the University of Illinois at Chicago. The animals were anesthetized by inhalation of isoflurane in a closed chamber before being moved to a surgical table. The surgical table was equipped with stereotaxis equipment and a nose cone to allow for administration of gaseous isoflurane during surgery. Once anesthetized, the hair on the back of the animal was shaved using hair trimmers. This allowed for obtain better signal acquisition during the FMT procedure. An L3 laminectomy was performed and the dura bone was thinned down till it could be penetrated using a 30G needle. A small volume of 0.17 mg mL-1 solution of the fluorescent nanocomposites was injected into the spinal subarachnoid space using a syringe pump. The imaging procedure described below was performed, afterwards rats were euthanized.
In vivo imaging procedure
FMT was performed using a Xenogen (Caliper Life Sciences) IVIS System equipped with anesthetic isoflurane system. The fluorescent nanocomposites required an excitation wavelength of 550 nm and the emission wavelength filter was set to 590 nm. Other settings used for image acquisition were: binning level= medium, fvalue = f/2 and acquisition time = 10 seconds.
To test the magnetic targeting capability of the nanocomposites, we developed a simple in vitro infusion system, in which amplitude and frequency of fluid pulsations can easily be adjusted. The model, as shown in Fig. 9, consists of a cylindrical polystyrene tube (49.0 cm length; 0.75 cm inner diameter) which encloses the fluid, which could be artificial CSF or water. A latex deformable membrane was tightly fixed to the mouth of the distal neck which expands and contracts to permit pulsatile motion of the fluid. Nanoparticle distribution inside the fluid varies depending on different patient specific parameters such as the frequency and magnitude of the pulsations, the velocity and stroke volume of fluid, and heart rate. In our lab, we are interested in the intrathecal delivery of the nanocomposites for treatment of CNS diseases. We therefore, imposed a fluid velocity of 1.5 cm s-1 and a stroke volume of 2 mL, which are within the average ranges for CSF movement, as cited in prior literature [58, 59]. Initially, we used water as the fluid in our system, because it has similar density to CSF . To produce pulsatile fluid motion equal in amplitude and frequency to that of a typical resting human’s CSF pulsations, the proximal end of the model was connected to a masterflex peristaltic pump (Cole-Parmer, IL, USA) and two of the three metal cylinders that drive the pump were removed. This created a ramping pulse function. Since CSF pulsates at a frequency of the heart beat, the pump was set at 70 beats per min which, according to the American Heart Association, is within the 60-80 bpm range of normal resting heart rate in humans. A predetermined volume of the nanocomposite solution (0.17 g mL-1) was injected into the model. A targeting magnet of 35 lb pull force strength was placed at a distance of 10 cm from the injection site to collect and localize the injected nanocomposites. This region represents the targeting region. The experiment lasted for 10 min during which the accumulation at the target magnet site was imaged. No observable increase in the collection efficiency of nanocomposites at the targeting magnet site was observed beyond 10 min.
Synthesis and characterization results gold-coated magnetite nanoparticles
The Fe3O4@Au MNPs were characterized using transmission electron microscopy (TEM), energydispersive xray spectroscopy (EDS), selected area electron diffraction (SAED) and superconducting quantum interference device (SQUID) magnetometry. The Fe3O4@Au MNPs used in our experiments had a Fe3O4 core with diameter between 812 nm (Fig. 1(a)) with a uniform gold shell thickness of 12 nm, making the total diameter of the particle to be around 20-25 nm (Fig. 1(b)). Fe3O4 cores of this size exhibit superparamagnetic property retaining no net magnetization when removed from an external magnetic field . They can be dispersed without agglomeration until an external magnetic field attracts the NPs, allowing for magnetic guidance and localization at the target site. The gold coating on the Fe3O4 core (Fig. 1(c)) serves two purposes: (i) It prevents the oxidation of the Fe3O4 core into maghemite (Fe2O3) by forming a protective coating layer. Fe2O3 has weaker magnetic susceptibility than Fe3O4 [62, 63], (ii) It also forms a convenient platform for loading drugs to the NP surface, since gold has a natural affinity for sulfur-containing functional groups and bonds. EDS was used to confirm the chemical composition of the nanoparticles. The spectra obtained indicated the presence of the elements gold (Au) and iron (Fe). The SAED patterns of the Fe3O4 cores and Fe3O4@ Au MNPs indicate ring patterns confirming the face centered cubic structure of both Fe3O4 and Au respectively. In Fig. 2(a), the spacings measured in the diffraction patterns of 0.30 nm, 0.265 nm, 0.218 nm and 0.150 nm are in agreement with the following standard reflections for Fe3O4: 0.297 nm (220); 0.253 (311); 0.210 nm (400); 0.148 nm (440). From the diffraction pattern shown in Fig. 2(b), the d-spacing is 0.236 nm. This is close to the closed packed Au (111) spacing of 0.2355 nm. These diffraction patterns confirm the Fe3O4 core and Au surface of our superparamagnetic nanoparticles. Determination of the magnetic properties of our nanoparticles was performed using SQUID magnetometry. From the plot of magnetization versus applied magnetic field (MH loop) at 265 K (Fig. 2(c)), the saturation magnetization (Ms), remnant magnetization (Mr), and coercivity (Hc) for the Fe3O4@Au MNPs were determined to be as follows: Ms = 29.5 emu g-1; Mr = 0.52 emu g-1; Hc = 21.1 Oe at 265 K. The hysteresis loop indicates no remnant magnetization, establishing the desired superparamagnetism of the NPs.
CdTe quantum dots
A microwave irradiation procedure was used to produce fluorescent, stable, water soluble CdTe QDs, which served as the functional imaging entities of our drug delivery nanocomposite vehicle. Red QDs (2-3nm) were synthesized with the procedure described in section 2.2. QDs of varying colors can by synthesized by this technique. The colors arise from the quantum confinement effect since different QD radii produce different emission wavelengths [51, 64, 65]. Size tunability was observed and confirmed through HRTEM analysis (Fig. 3(a), 3(b)). Fluorescence emission of the red QDs was confirmed using a fluorescence spectroscopy (Thermo Scientific NanoDrop 3300 fluorimeter). The absorption and fluorescence emission spectra of the QDs, shown in Fig. 3(d) and 3(e), indicate that the QDs have absorption and emission maxima peaks at 550 nm and 590 nm respectively. Red CdTe QDs were chosen for the synthesis of the nanocomposites because they have an emission peak of 590 nm, which is better-suited for in vivo imaging.
QD-Fe3O4@Au fluorescent nanocomposite
The nanocomposites had a total diameter of around 30-45 nm, which consisted of a Fe3O4@Au NP core conjugated to QDs on its surface (Fig. 4(a)). These nanocomposites are superparamagnetic, because the Fe3O4@Au MNPs that form the core of the structure are superparamagnetic. This property prevents agglomeration during delivery. The stability of QDs after loading on the surface of the nanocomposites was studied with fluorescence and UV-Vis spectroscopy. When red QDs were loaded onto the MNP surface, the nanocomposites showed similar absorption and emission peaks as the plain red CdTe QDs, with the emission peak at 590 nm. No change in the fluorescence spectral profile of the QD-Fe3O4@Au nanocomposites was observed when compared to the plain QDs (Fig. 5(a)). FMT was also used to confirm the fluorescence emission of the conjugated structure in several environments, as shown in Table 1 and Fig. 4(d), 4(e). In order to determine the fluorescence emission stability of the nanocomposites over an extended period of time, the nanocomposite solution was kept in the dark at room temperature and small samples were withdrawn at regular intervals to examine the fluorescence emission. A negligible loss of fluorescence was observed over a period of three weeks. After that time, fluorescence decayed exponentially (Fig. 5(b)), due to gradual oxidation of the CdTe QDs. Complete loss of fluorescence was observed after two months.
Cellular uptake experiment
Confocal microscopy images (Fig. 6(b)) indicate the presence of the QD-Fe3O4@Au nanocomposites inside a C6 glioma cell. Insets B1 and B2 show z-stack images (x-z and y-z respectively) of the cell confirming the presence of the nanocomposites inside it. Fig. 6(a) shows fluorescence images of the nanocomposites transfected into rat astrocyte cells. The cell uptake experiment results (Fig. 7(a)) show that these nanocomposites are readily taken up by the cells. The C6 glioma cell uptake was quantified by using fluorescence measurements to understand the transfection of nanocomposites into the cells. A trend of increasing cellular uptake was observed as the incubation concentration of nanocomposites was increased (Fig. 7(a)).
Enhancement of cellular uptake using magnetic fields
Cell uptake of the nanocomposites in the presence of magnetic fields was studied. C6 glioma cells were incubated with a 0.3125 mg mL-1 solution of nanocomposites for 1 h in the presence of a magnetic field generated by a 3.23 lb pull force permanent magnet. Fig. 7(b) shows a 5-fold increase in the nanocomposite uptake by the cells in the presence of the magnetic field (N = 5). Similarly, a 300400% increase in transfection was observed when solutions of 0.625 mg mL-1, 1.25 mg mL-1, and 2.5 mg mL-1 concentrations were used in the presence of magnetic fields (data not shown).
Magnetic drug targeting
MDT of our nanocomposites can be achieved by administration via the blood or CSF, both fluids move in a pulsatile manner in the body. To prove magnetic localization, the MDT experiment was conducted as mentioned in §2.7 to demonstrate magnetic targeting of our nanocomposites. It was repeated twice, with injection volumes of 1mL and 0.5 mL of 0.17 g mL-1 nanocomposite solution, as shown in Fig. 8(b) and 8(c) respectively. Digital photo images were acquired every 2 min to observe the nanocomposite localization at the targeting magnet site. The QD-Fe3O4@Au MNP solution can be observed with normal light due to ts dark brown color. Both experiments indicate an increase in the accumulation of the nanocomposites with time, despite the mixing effect of the pulsatile motion of the fluid. The experiment was stopped at the 10 min time point, because no further increase in nanocomposite collection was observed. Maximum accumulation occurred at the edges of the magnet, as the field strength is the greatest at this region. Almost no nanocomposites were found to cross the magnet and move towards the peristaltic pump, as can be seen in frame 6 of Fig. 8(b). Such a barrier created by the magnetic field is very useful for drug delivery treatments where a cytotoxic drug should be confined to a target zone, such as a spinal tumor, without spreading to other regions. During both experiments, at least 80% of the nanocomposites were collected at the target magnet site, with larger accumulation observed for the smaller injection volume. Magnet size, shape and field strength can be modulated depending on required drug dosage for therapeutic efficacy and patient convenience.
In vivo imaging results
To demonstrate live imaging capability, the fluorescence emission of these nanocomposites was measured through both rat muscle and bone tissue. 200 μL of the nanocomposite solution (2.5 mg mL-1) was injected into the abdominal muscle of a Sprague Dawley rat at a depth of 1 cm from the skin surface. FMT images were generated using the IVIS® system. Fig. 9(a), 9(b) shows that the nanocomposites are clearly visible, demonstrating that deep tissue imaging is feasible using this drug delivery vehicle. The BBB creates a great obstacle for effective drug delivery to the CNS [66, 67]. To show the ability to track our drug delivery vehicle inside the CNS of animals, these particles were intrathecally injected into the lumbar region of a rat. Fig. 9(b), 9(c) shows the images generated from the IVIS® system when small volumes of the nanocomposites were injected into the spinal subarachnoid space (at the L3 region) of the rat. The fluorescence maps indicate that visualization is possible through bone and tissue of the spine. The fluorescence emission of the nanocomposites in all environments, especially in in vivo environments is significant, indicating its applicability for in vivo drug delivery purposes, as shown in Table 1.
Fig. 1 Characterization of Fe3O4@Au MNPs (a) Schematic of Fe3O4@Au NP; (b) TEM image of Fe3O4@Au MNPs showing the total diameter of the particle to be between 20-25 nm; (c) Schematic of Fe3O4@Au NP that has been partially coated with gold (d) TEM image (corresponding to schematic shown in Fig. 1(c)) that clearly delineates the Fe3O4 core and Au coating. This image confirms the length of core radius (4-6 nm); (e) EDS spectrum of Fe3O4@Au MNPs which confirms the presence of elements Au, Fe and O; (f) TEM image showing multiple Fe3O4@Au MNPs along with a histogram of their size distribution.
Fig. 2 Characterization of Fe3O4@Au MNPs (a) SAED pattern of the Fe3O4 core; (b) SAED pattern of the Fe3O4@Au MNPs; (c) M-H loop (magnetization versus applied magnetic field) obtained by SQUID magnetometry at 265 K.
Fig. 3 Characterization of CdTe QDs (a) TEM image of red QDs measuring about 2-3 nm. Some of the QDs have been circled for better visibility; (b) Histogram showing the size distribution of the red QDs; (c) Absorption spectra of the CdTe QDs with a peak at 550 nm; (d) Emission spectra of the CdTe QDs showing a peak at 590 nm. An inset has been included which shows FMT image of QDs injected into a 6% agarose gel.
Fig. 4 Characterization of QD-Fe3O4@Au nanocomposites (a) Schematic of QD-Fe3O4@Au nanocomposite; (b) TEM image of the nanocomposite that shows both the nanocomposite core (white circle) as well as the conjugated QDs (smaller pink circles); (c)Enlarged view of the nanocomposite surface showing the individual QDs (pink circles); (d) FMT images of the QD-Fe3O4@Au nanocomposites injected in a 6% agarose gel; (e) Normal and FMT images of the nanocomposite solution.
Fig. 5 Fluorescence characterization of QD-Fe3O4@Au nanocomposites (a) Emission spectra of the QD-Fe3O4@Au nanocomposites (orange) and plain CdTe QDs (blue). Maximum emission wavelengths for both were determined to be 590 nm; (b) Graph showing the loss of fluorescence of the QD-Fe3O4@Au nanocomposites over time.
Fig. 6 Fluorescence microscopy results showing uptake by rat cortical astrocytes and C6 glioma cells on incubation in a 0.034 mg mL-1 QD-Fe3O4@Au NP solution for 30 min. All cells have been stained labeled using DAPI, which stains the nucleus blue, and phalloidin, which stains the actin cytoskeleton green. The nanocomposites appear red corresponding to the emission wavelength of 590 nm. (a) Image showing nanocomposite localization within rat astrocyte cells. Insets contain images of separate stains: DAPI, QD-Fe3O4@Au MNPs and phalloidin; (b) Confocal microscopy images of QD-Fe3O4@Au MNPs transfected into C6 glioma cells. Insets B1 and B2 show the z-stack images (x-z and y-z) indicating the presence of nanocomposites inside the cell.
Fig. 7 Results of cell uptake and cytotoxicity studies using C6 glioma and rat cortical astrocyte cells (a) Fluorescence quantification of nanocomposite uptake in C6 glioma cells. A spike in nanocomposite uptake is observed at a lower concentration of nanocomposite solution (2.5 mg mL-1). (b) Uptake of nanocomposites (0.3125 mg mL-1) in C6 glioma cells under the influence of a magnetic field. Increased transfection occurs under the influence of a 3.23 lb pull force magnetic field.
Fig. 8 In vitro magnetic drug targeting experiment (a) Schematic of the experimental setup containing the injection site, targeting magnet and peristaltic pump to create the pulsatile motion of the fluid; (b) (c) Images of the MDT experiment showing localization of the nanocomposites at the target site for an injection volume of (b) 1 mL and (c) 0.5 mL.
Fig. 9 In vitro imaging of our nanocomposites using IVIS® system in a Sprague Dawley rat. (a) FMT image of the QD-Fe3O4@Au nanocomposites (200 μL) injected into the abdominal muscle of a rat at a depth of 1 cm; FMT image of the QD-Fe3O4@Aunanocomposites injected into the intrathecal space of a Sprague Dawley rat (c) Injection volume = 200 μL; (d) Injection volume = 50μL.
Table 1 Epifluorescence measurements made from the nanocomposites in different environments
Our results show the successful synthesis and characterization of a novel drug delivery vehicle for MDT that can be visualized in vivo during delivery to the targeted disease site. The magnetic core of the nanocomposite is superparamagnetic, therefore, targeting drugs using this vehicle via MDT is possible. External magnetic fields can arrest the nanocomposites within any targeted region, despite the convective transport and mixing effect caused by the pulsatile motion of CSF as shown by the in vitro MDT experiment. Moreover, the cellular uptake experiments demonstrated internalization of nanocomposites in tumor cells. The vehicle was successfully visualized various environments (cellular, in vitro and in vivo) using FMT techniques, offered by commercial imaging equipment such as the IVIS® system (Caliper Life Sciences). All the necessary attributes for an MDT drug delivery vehicle have been attained in our nanocomposites, such as magnetic guidability and in vivo imageability. Drug functionalization can also be achieved due to the affinity that gold has for sulfurcontaining functional groups and bonds (e.g. thioland mercapto bonds), which are found in many proteins, pharmaceutical and biopharmaceutical drugs. Unlike previous studies which suffered fluorescence quenching after gold surfaces conjugation [68, 69], our QD conjugation retained strong fluorescence emission even after conjugation to the gold-coated magnetic nanoparticle. Fluorescence emission from our nanocomposite structure was stable over a three-week period of time, demonstrating its suitability for longer term experiments. The gold and Fe3O4 of our nanocomposites are biocompatible. Fe3O4 degrades into its components and can be integrated in normal iron homeostasis, without posing a threat [70, 71]. A disadvantage of our system is that the CdTe QDs are not biocompatible. It was observed that concentrations of upto 20 mg/mL of our nanocomposites containing CdTe QDs were taken up by cells over a period of 5 h. However, higher concentrations would lead to toxic effects. One of the key steps in our future work will be to synthesize CdTe QDs with a shell of another compound such as ZnS to prevent release of Cd2+ ions, which has a detrimental effect on cells . Our CdTe QDs capped with thiol bonds are synthesized using 3 mercaptopropionic acid. Another possibility would be to use a different stabilizer during the synthesis process, such as tripeptide glutathione (GSH) instead of the 3 mercaptopropionic acid. This would results in biocompatible CdTe QDs capped with GSH group . The GSH group contains a sulfur atom, and QDs capped with GSH can be loaded on the gold surface of our nanocomposite using a similar technique as mentioned in §2.3. GSH can be used to detoxify Cd2+ ions in medicine due to its chelating capability . We believe that for MDT, the drug dosages used would be much lower compared to systemic delivery, as the therapeutic molecules can be specifically delivered at target sites. Therefore, a very high concentration of our nanocomposites would not be used for drug delivery. Also, in vivo studies suggest that, regardless of the nature of the QDs, vertebrate systems tend to recognize them as foreign, with rapid elimination of the materials through the primary excretory-organs/ systems: the liver, spleen, and lymphatic systems . Once inside the target cell, gold-thiol bonds are rapidly broken due to the reducing environment of the cell cytoplasm, and the QDs will be released from the Fe3O4@Au NP core. QDs of sizes similar to the ones that we use can be easily and efficiently cleared from the body . Specific biocompatibility studies would be needed for the definite concentration ranges that are safe for therapeutic application.
This nanovehicle has possible MDT applications for the treatment of gliomas, spasticity, pain and other diseases where focal targeting and in vivo visualization are required. It can also be applied in CNS disease treatments where guidance to the target site has proven to be a major challenge. MDT is an effective technique to achieve localized drug action without systemic toxicity within the CNS. Future work would involve increasing the biocompatibility of our nanocomposites by utilizing techniques such as synthesis of core-shell QDs that contain an inner core of CdTe and an outer shell of a more biocompatible material such as ZnS or ZnTe. Our future research would also involve conjugating drug molecules to the nanocomposite surface followed by in vivo validation of MDT using an animal model. Such multifunctional nanoparticles hold great promise for targeted disease treatment.
TF and DA would like to acknowledge their fellowships from the National Science Foundation-Research Experience for Teachers (NSF-RET) Program.
This work was supported in part by the National Science Foundation Grant-CBET 1010621 and the NSF RET EEC 1132694.
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Copyright© 2016 Indu Venugopal, Sebastian Pernal, Taylor Fusinatto, David Ashkenaz and Andreas Linninger. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.