Development of Sars-Cov-2 Isothermal Amplification Detection Kits

Xueling Li, Jing Tian, Yan Xu, Xiaomin Chen, Zexi Liu, Hui Liang, Caihong Jin, Daxiang Cui

Abstract

The Sars-CoV-2 isothermal amplification detection kits based on loop-mediated isothermal amplification (LAMP) were developed and evaluated on three types samples of Sars-CoV-2. The kits included enzyme reaction mixtures and chromogenic agents. After the isothermal amplification reactions were completed, the reaction results were judged by using the chromogenic agents to determine whether Sars-CoV-2 exists in the samples to be tested. The detection kits have the advantages of convenient operation, fast detection speed and high sensitivity up to 1 copy of virus particles per reaction, which can speed up the detection speed of suspected cases, and avoid the missing detection problems caused by the low detection sensitivity.

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Nano Biomedicine and Engineering.

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