Preparation of Aluminum Oxide Nanoparticles by Laser Ablation and a Study of Their Applications as Antibacterial and Wounds Healing Agent

Aluminum oxide nanoparticles were prepared and characterized with a wide variety of applications. This study aimed at the preparation of AlO₂ nanoparticles by the liquid particles treated with laser (1064 nm), immersed in deionized water at varied laser energy (350 J/cm²) and diverse numbers of 300 pulse. For antimicrobial, antioxidants, animal's model, the prepared nanoparticles were confirmed by using ultraviolet–visible spectroscopy (UV-Vis), X-ray diffraction (XRD) and scanning electronic microscope (SEM). The antimicrobial activity of synthesized Al₂O₃ nanoparticles was tested using well diffusion method against three types of bacteria: Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The best concentration of aluminum oxide nanoparticles was 100 mg/mL, which exhibited a higher antibacterial activity against both gram-negative and -positive bacteria. In addition, the results showed that the gram-negative bacteria were more resistible than gram-positive bacteria. The results also indicated that the aluminum oxide nanoparticles possessed antioxidant effects, as investigated by DPPH method. Finally, the wound healing activity of the aluminum oxide nanoparticles was investigated, and the results showed that the aluminum oxide nanoparticles stimulated the wound healing activity by using animal's model. The results presented in this study demonstrated that the aluminum oxide nanoparticles had biomedical applications could be applied in biomedical uses in the future.