Preconcentration and Determination of Pantoprazole by Solid-phase Extraction Coupled with Spectrophotometry Using Iron Oxide Nanoparticles Modified with Cetyltrimethylammonium Bromide

Solid phase extraction coupled with spectrophotometric detection was applied to trace amountsof Pantoprazole (PP) drug using Cetyltrimethylammonium bromide coated-iron oxide magnetitenanoparticles CTAB@Fe3O4 MNPs. After characterization of the prepared nano-adsorbents,experimental parameters affecting the extraction efficiency of the developed method were optimized.The results obtained showed that this proposed approach is applicable in concentrations ranging from0.1 to 1.5 μg/ml (R = 0.9958) indicating that follows Beer’s-Lambert law. The limit of detection andthe limit of quantification calculated to be 0.014 and 0.04 μg/ml, respectively. The repeatability of theproposed method was evaluated with studying intra-day and inter-day precisions described by relativestandard deviations. Eventually, two samples of natural waters as well as samples of human plasmawere analyzed using the proposed method. Satisfactory precision and recoveries in the complexmatrices were achieved.